Work

Generation of an induced pluripotent stem cell line, LCSBi021-A, from a Parkinson’s disease patient harboring the missense mutation c.213C>G (p.Ser71Arg) in the RAB32 gene

Year: 2026

Type: preprint

Abstract: Dermal fibroblasts were obtained from a 40-year-old male patient with Parkinson’s disease (PD), harboring the c.213C>G (p.Ser71Arg) variant in the RAB32 gene. Induced pluripotent stem cells (iPSCs) were generated using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). The presence of the c.213C>G base exchange in exon 1 of RAB32 was confirmed by Next Generation Sequencing. The iPSCs are free of genomically integrated reprogramming genes, express pluripotency markers, display in vitro differentiation potential into all three germ layers and exhibit karyotypic integrity. Our iPSC line is available for research purposes to dissect molecular mechanisms of neurodegeneration in PD.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.10.20.760406

Authors: Ioanna Boumpoureka, Vyron Gorgogietas, Elizabet Petkovski, Rashi Halder, François Massart, Rejko Krüger

Institutions: Luxembourg Institute of Health, Luxembourg Centre for Systems Biomedicine, Laboratoire National de Santé, Centre Hospitalier de Luxembourg

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Université du Luxembourg, European Commission, Fonds National de la Recherche Luxembourg, HORIZON EUROPE Framework Programme