Asymmetry-induced distinct mechanisms and the transporting role of sodium in bacterial fluoride channel Fluc
Year: 2026
Type: article
Abstract: Bacterial fluoride channels (Fluc) are exceptional in their ability to conduct fluoride ions at rapid rates while maintaining extraordinary selectivity, an unusual property among anion transporters. Fluc achieves this through an antiparallel homodimeric assembly that forms two asymmetric ion-conduction pores and hosts a centrally bound sodium ion of previously uncertain function. Despite extensive structural characterization, the molecular basis for Fluc's dual-pore asymmetry, transport efficiency, and sodium involvement has remained unresolved. Here, we performed long-timescale molecular dynamics simulations under electrophysiological conditions to elucidate the mechanisms of fluoride translocation through each pore. Our results reveal two distinct conduction modes: Pore I operates via the Channsporter mechanism, characterized by single-ion transport; while Pore II follows the Multi-ion mechanism in which paired fluoride ions exploit electrostatic repulsion to achieve faster sequential translocations. These findings demonstrate that structural asymmetry gives rise to mechanistic specialization within the same homodimeric protein. We further propose that the central sodium ion serves as a dynamic cofactor in fluoride transport, coupling its vertical oscillation to fluoride movement in a pore-dependent manner. Together, our results establish a unified model of asymmetric dual-pore conduction in Fluc and introduce a broader paradigm in membrane transport biology, one where inherent structural asymmetry enables divergent yet coexisting mechanisms of ion conduction.
Source: Nature Communications
Institution: The University of Texas at Dallas
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
OpenAlex ID: W7223415891