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Immune modulation by lipopolysaccharide preconditioning: A novel approach to neuropathic pain in rats

Year: 2026

Type: article

Abstract: Background: Neuropathic pain (NPP) resulting from peripheral nerve compression is associated with heightened nociceptive sensitivity and increased neuroinflammation. This study investigated the effects of lipopolysaccharide (LPS) preconditioning on behavioral and inflammatory outcomes in a rat model of the chronic constriction injury (CCI)-induced NPP. Material and methods: Animals were assigned to groups including Control, CCI (underwent CCI surgery), LPS (0.01 mg/kg) + CCI, LPS (0.1 mg/kg) + CCI, and LPS (0.5 mg/kg) + CCI, which received LPS intraperitoneally and then underwent CCI surgery. Pain-related behaviors were assessed using the Acetone Test and Hot Plate Test on days 7 and 14 after CCI induction. Then, Spinal cord samples were collected to quantify tumor necrosis factor alpha (TNFα) levels, a key pro-inflammatory mediator. Result: CCI induction significantly increased hypersensitivity to pain, accompanied by elevated TNF-α expression in the spinal cord. LPS preconditioning attenuated pain behaviors and reduced TNF-α levels compared with the CCI group. Conclusion: These findings suggest that LPS preconditioning modulates neuroimmune responses and may confer protective effects against NPP development induced by peripheral nerve compression.

Source: IBRO Neuroscience Reports

DOI: 10.1016/j.ibneur.2026.10.006

Authors: Soqra Hesam, Fatemeh Piri, Mahshid Moradpour, Hamid Sepehri

Institutions: Golestan University, Golestan University of Medical Sciences

Language: English

Topic: Pain Mechanisms and Treatments

Subfield: Physiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,400