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The Effects of Ampicillin versus Tetracycline in the Selection of pBR322 Transformants

Year: 2026

Type: article

Abstract: Escherichia coli DH5 α cells were transformed with pBR322 plasmids, which encode both ampicillin and tetracycline resistance genes. After 45- and 60-minute recovery periods, the transformed cells were plated on three types of selective plates, containing 1) ampicillin only, 2) tetracycline only, and 3) ampicillin plus tetracycline. It was found that tetracycline selection from these recovery periods resulted in a lower level of transformant survival than ampicillin selection. However, after the transformants were allowed to recover for several generations, their survival rates were similar on ampicillin plates and on tetracycline plates.

Source: Open Collections

DOI: 10.14288/ujemi.v2.204255

Authors: LAW, JOYCE, LEE, SHARON, TSENG, AMY, TSUI, KO WING, YU, NANCY

Institution: University of British Columbia

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7223472018

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