Genomic in vitro transcription and Nanopore direct RNA sequencing of a human B-Lymphocyte cell line
Year: 2026
Type: article
Abstract: Genomic DNA used as a template for in vitro transcription of RNA can serve as a true negative control for benchmarking RNA modification detection by Nanopore direct RNA sequencing (DRS) models. We generated DRS data for in vitro transcribed (IVT) RNA composed of canonical nucleotides using genomic DNA from a human cell line. We applied Dorado modification calling models to these data, and calculated 9-mer specific false-positive rates for eight RNA modifications as a comparison point for future development of RNA modification models.
Source: GigaScience
Institution: Northeastern University
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,638
Funder: National Institutes of Health
OpenAlex ID: W4409865845