Tissue-Specific Regulation of Postprandial Non-Esterified Fatty Acid Metabolism in individuals with prediabetes
Year: 2026
Type: preprint
Abstract: After a meal, it is generally assumed that tissue non-esterified fatty acids (NEFA) uptake falls in proportion of the reduction of systemic NEFA appearance from insulin-mediated suppression of adipose tissue lipolysis. Here, we combined dynamic [ 11 C]palmitate PET/CT, stable isotope tracers, and MRI to measure palmitate uptake, oxidation, and esterification in the heart, liver, skeletal muscle, and subcutaneous and visceral adipose tissue, in the fasted state and 60min after a mixed meal in individuals with prediabetes. Net palmitate uptake decreased in all tissues, but most tissues extracted a larger fraction of circulating palmitate after the meal. The intracellular fate of palmitate differed between organs. The heart shifted toward oxidation, the liver toward esterification, maintaining VLDL-palmitate release, and this shift was smaller in individuals with more liver fat. Both adipose depots increased fractional esterification, and visceral fat almost stopped releasing palmitate. Thus, plasma NEFA overestimates the postprandial reduction in lean tissue NEFA exposure. Highlights After a meal, net palmitate uptake fall in all tissues but extraction rises in most After a meal, the liver directs palmitate toward esterification and the heart toward oxidation Plasma NEFA level underestimate lean tissue NEFA exposure after a meal in prediabetes
Source: medRxiv
Authors: Elisa Le Roux, Sophie Hogan-Lamarre, Marie Lepitre, Frédérique Frisch, Lauralyne Dumont, Mélanie Fortin, Gabriel Richard, Christophe Noll, Otman Sarrhini, Warner Mampuya, Serge Phoenix, Lucie Bouffard, Brigitte Guérin, Éric E. Turcotte, Femke Rutters, Parminder Raina, Jean-Pierre Desprès, Joris Hoeks, Patrick Schrauwen, André C. Carpentier, Denis P. Blondin
Institutions: Université de Sherbrooke, Centre de recherche du CHUS, Amsterdam Public Health, Amsterdam UMC Location Vrije Universiteit Amsterdam, McMaster University, Université Laval, VITAM - Centre de recherche en santé durable, Maastricht University, Deutsches Diabetes-Zentrum e.V., Heinrich Heine University Düsseldorf, Leiden University Medical Center, German Center for Diabetes Research
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7223693174