Work

Substrate recognition and stage-dependent transformation of aflatoxin B1 by mediator-free Bacillus laccase frL103

Year: 2026

Type: article

Abstract: 327.0504 was putatively assigned as Dehydro-AFQ1, a proposed dehydrogenated AFQ1-related product. The 60 h reaction mixture produced higher viability than intact AFB1 in HepG2 and 293T cells, structure-based predictions retained endpoint-dependent hazard signals for the proposed products. In molecular dynamics simulations, AFB1 and its proposed products differed in hydration, distance distributions relative to the T1 copper center and binding-pocket retention. These findings establish a stage-resolved, product-centered framework for evaluating enzymatic AFB1 transformation. This approach links bioprocess performance with product evolution, cytotoxicity attenuation, and substrate recognition, while identifying the need for product confirmation, quantitative mass balance.

Source: RSC Advances

DOI: 10.1039/d6ra07811g

Authors: Luyao Bian, Ting Wang, Feiyang Pan, Jinwen Cai, Xu Li, Chong Zhang

Institution: Nanjing Agricultural University

Language: English

Topic: Mycotoxins in Agriculture and Food

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,042

Funders: Nanjing Agricultural University, Priority Academic Program Development of Jiangsu Higher Education Institutions, Fundamental Research Funds for the Central Universities