Structural analysis of protein machineries using single particle cryo-EM
Year: 2026
Type: dataset
Abstract: Cryogenic single-particle electron microscopy (Cryo-EM) is the method of choice for determining the structures of large macromolecular complexes and membrane proteins – targets that have been challenging for X-ray crystallography. Exciting recent advances in cryo-EM now allow for structure determination at high resolution, of proteins in multiple states, of smaller proteins and of membrane proteins within a natural lipid environment. In this BAG, we will use the state-of-the-art cryo-EM facilities at the ESRF to determine the structures of intricate protein machineries including large multi-subunit complexes, protein-nucleic acid complexes and membrane protein complexes. Our projects will contribute to a better understanding of essential cellular processes such as energy conversion and cellular respiration, nitrate and ammonium metabolism, ribosome and ribosome-associated functions, and DNA damage repair and maintenance mechanisms.
Authors: Hunte, Carola, Kumar, Anil
Institutions: University of Freiburg, University of Würzburg
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
Award: G799273300
OpenAlex ID: W7224123646