MYL9 silencing inhibits proliferation and migration of lung adenocarcinoma cells: a potential therapeutic target
Year: 2026
Type: article
Abstract: Background: Myosin light chain 9 (MYL9), a regulatory light chain of myosin II, plays a crucial role in cytoskeletal dynamics and has been associated with tumor progression in multiple malignancies. However, its role in lung adenocarcinoma (LUAD) remains poorly characterized. Despite recent advances in treatment, the prognosis of LUAD patients remains poor, underscoring the need for novel therapeutic targets.Methods: Here, we systematically assessed MYL9 expression and its clinical relevance in LUAD by integrating transcriptomic data from The Cancer Genome Atlas (TCGA) and protein-level validation from the Human Protein Atlas (HPA) via immunohistochemistry. Functional investigations in H1299 cells indicated that silencing MYL9 significantly impeded cell proliferation, migration, and invasion, as corroborated by colony formation and Transwell assays. In vivo xenograft models further verified that MYL9 knockdown inhibited tumor growth and triggered apoptosis. Bioinformatic analyses, including the ESTIMATE algorithm, single-sample gene set enrichment analysis (ssGSEA), and drug sensitivity profiling, were also performed.Results: In addition to its oncogenic impacts, bioinformatic analyses demonstrated that elevated MYL9 expression is correlated with distinct immune infiltration patterns and diminished chemosensitivity, possibly through the modulation of the tumor microenvironment. These findings suggest that MYL9 may link cytoskeletal regulation with tumor progression, immune remodeling, and treatment response in LUAD.Conclusions: Collectively, these findings recognize MYL9 as a novel prognostic biomarker and therapeutic target in LUAD, providing insights into its multifaceted role in tumor biology and treatment response. Further studies are warranted to validate these findings in larger clinical cohorts and to elucidate the underlying molecular mechanisms.
Source: Journal of Thoracic Disease
Language: English
Topic: Lung Cancer Research Studies
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7224126381