Work

Protocol for low-input whole-genome bisulfite sequencing in Arabidopsis

Year: 2026

Type: article

Abstract: DNA methylation profiling of rare plant samples is limited by the scarcity of input DNA. Here, we present a protocol for low-input whole-genome bisulfite sequencing (WGBS) in Arabidopsis in which DNA inputs as low as 50 pg can generate methylomes comparable to conventional WGBS. We describe steps for tissue cryomilling, Proteinase K lysis, and direct bisulfite conversion. We then detail procedures for post-bisulfite adapter tagging library construction and MethylStar-based alignment and methylation calling.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104928

Authors: Jie Yang, Binh Thanh Vo, Frank Johannes

Institution: Technical University of Munich

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Bundesministerium für Bildung und Forschung, China Scholarship Council