Work

Expanding the conjugation toolbox of DNA-stabilized silver nanoclusters with biotin-streptavidin

Year: 2026

Type: article

Abstract: cluster. Incorporation of biotin either at 3' end or in the middle of the sequence, enabled selective immobilization of the resulting biotinylated DNA-AgNCs on streptavidin-coated poly(methyl methacrylate) (PMMA) microparticles. Importantly, biotin functionalization and subsequent particle attachment largely preserved the characteristic optical properties and fluorescence lifetimes of the DNA-AgNCs, while control experiments showed negligible nonspecific binding of the non-biotinylated DNA-AgNCs. These results demonstrated that biotin-streptavidin interaction provides a robust strategy for the modular functionalization of DNA-AgNCs without compromising their photophysical properties, opening opportunities for their integration into future bioimaging and sensing applications.

Source: RSC Advances

DOI: 10.1039/d6ra09400g

Authors: Vanessa Rück, Simon Wenzel Lind, Christian Brinch Mollerup, Tom Vosch

Institution: University of Copenhagen

Language: English

Topic: Nanocluster Synthesis and Applications

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,042

Funders: Natur og Univers, Det Frie Forskningsråd, Villum Fonden, Novo Nordisk, Novo Nordisk Fonden