Work

A substituted cysteine accessibility protocol to probe orthosteric and non-canonical ligand binding to ion channels

Year: 2026

Type: article

Abstract: The Substituted Cysteine Accessibility Method (SCAM) is an approach for probing the biophysics and pharmacology of ion channels by targeting covalent modifications to individual amino acids. Here, we present a protocol to constrain channel stoichiometry and identify asymmetric or non-canonical ligand-binding sites using SCAM followed by electrophysiology in mammalian heterologous expression systems, in the context of heteropentameric nicotinic acetylcholine receptors. This protocol is applicable to examination of multiple channel properties, including ligand binding, gating, pore conductance, and selectivity. For complete information on the use and execution of this protocol, please refer to Kulkarni et al. 1 1. Kulkarni, G.C. ∙ Jurek, M.J. ∙ Riley, A.P. ... An accessory orthosteric ligand binding site in the (α3)3(β4)2 nicotinic acetylcholine receptor regulates channel activation Cell Rep. 2026; 45 , 117077 Full Text Full Text (PDF) Scopus (0) Google Scholar

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104934

Authors: Gauri C. Kulkarni, Christian J. Peters

Institution: University of Illinois Chicago

Language: English

Topic: Nicotinic Acetylcholine Receptors Study

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funder: National Institute on Drug Abuse