Work

Single‐molecule force spectroscopy of biomolecules using AFM. A multigenerational perspective

Year: 2026

Type: review

Abstract: Single-molecule force spectroscopy (SMFS) using atomic force microscopy (AFM) allows the direct application and measurement of forces on individual molecular systems; revolutionizing our understanding of biomolecular mechanics and receptor-ligand interactions and opening the field of single-molecule mechanobiology. This review traces the evolution of the field, from foundational landmark experiments to recent developments that allowed revisiting those seminal works. We discuss the critical technological advancements, functionalisation strategies and theoretical developments that have shaped the discipline. Finally, we suggest future directions to further elucidate the intricate relationship between structure and mechanical properties of single biomolecules.

Source: Journal of Microscopy

DOI: 10.1111/jmi.70196

Authors: Ismahene Mesbah, Claire Valotteau, Felix Rico

Institutions: Inserm, Aix-Marseille Université, Dynamique et Nanoenvironnement des Membranes Biologiques

Language: English

Topic: Force Microscopy Techniques and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,760

Funders: Institut National de la Santé et de la Recherche Médicale, European Research Council