Work

Cross‐Platform Concordance Between Metagenomic and Targeted DNA Sequencing for Diagnosing Polymicrobial Infections in an Immunosuppressed Patient: A Case Report

Year: 2026

Type: article

Abstract: ABSTRACT Immunocompromised patients are highly susceptible to complex and rapidly progressive infections, for which early pathogen identification is critical. We report the case of an immunosuppressed patient aged 40 years with severe mixed pulmonary infections caused by Pneumocystis jirovecii and carbapenem‐resistant Klebsiella pneumoniae . During the disease course, metagenomic next‐generation sequencing and targeted next‐generation sequencing were repeatedly performed using three sequencing platforms. All platforms consistently identified the pathogens, guiding timely adjustment of antimicrobial therapy. Compared with conventional cultures (first positive result on hospital day 10), next‐generation sequencing detected Pneumocystis jirovecii 9 days earlier and rising Klebsiella pneumoniae 1 day before culture positivity and septic shock. This case highlights the clinical utility of metagenomic next‐generation sequencing and targeted next‐generation sequencing, providing real‐world evidence of cross‐platform concordance.

Source: Medicine Advances

DOI: 10.1002/med4.70091

Authors: Mengxia Wang, Jielun Wen, Liyue Sun

Institutions: Jinan University, First Affiliated Hospital of Jinan University

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: National Natural Science Foundation of China