Preparation of dual-ligand magnetic hydrogen-bonded organic frameworks and their application for enrichment of glycosylated and phosphorylated peptides
Year: 2026
Type: article
Abstract: @HOF successfully captured 29 distinct glycopeptides and 24 phosphopeptides，as identified by MALDI-TOF MS sequencing. From human serum samples，five typical endogenous phosphopeptides were unambiguously detected. These results confirm the reliability and accuracy of the method for real clinical samples and highlight its potential for large-scale PTM analysis in clinical proteomics，especially for profiling of glycosylation and phosphorylation. In summary，the dual-ligand magnetic HOF offers a powerful platform for high-specificity enrichment of both glycopeptides and phosphopeptides from complex biological matrices. Its excellent performance in clinical samples demonstrates its promise as a key tool for advancing clinical proteomics research，with potential applications in disease biomarker discovery and diagnostic support.
Institution: Jilin University
Language: Chinese
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7225167310