Work

In vitro NAD + supplementation increases CD4 + and CD8 + T cell function in people with HIV

Year: 2026

Type: article

Abstract: OBJECTIVES: HIV perturbs T cell homeostasis and disrupts immune function, increasing the risk of age-associated comorbidities. Ageing people with HIV (PWH) have exacerbated expression of CD38 on T cells, which depletes intracellular nicotinamide adenine dinucleotide (NAD+). We investigated whether boosting intracellular NAD+ could restore T cell homeostasis and function. METHODS: We collected blood samples from virally suppressed PWH to determine the effects of in vitro NAD+ on CD38 expression and T cell function in response to anti-CD3/CD28 antibodies stimulation in PBMCs culture supplemented with IL-2 and IL-7. RESULTS: We observed that NAD+ supplementation not only reduced expression of CD38 but also improved T cell function with an alleviated exhaustion phenotype in response to anti-CD3/CD28 antibodies stimulation. CONCLUSIONS: If confirmed in clinical trials, NAD+ supplementation has the potential to restore T cell homeostasis and immune function in PWH and ameliorate age-associated comorbidities.

Source: HIV Medicine

DOI: 10.1111/hiv.70342

Authors: Guangming Li, Salma Sharaf, Yaoxian Lou, Lishan Su, Shyam Kottilil, Poonam Mathur

Institution: University of Maryland, Baltimore

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,150