Year: 2026
Type: preprint
Abstract: Pre-fertilization barrier is the primary cause of intersubgeneric hybridization barrier in waterlily. The research results indicate that reactive oxygen species (ROS) are involved in regulating the germination process of pollen on the stigma of waterlily after pollination, and candidate genes NcSnRK1 and NcRbohC1 have been identified; Transient transgenic experiments were used to validate the functions of NcSnRK1 and NcRbohC1 on the waterlily stigma. Through yeast two-hybrid assays, luciferase complementation, and Pull-down experiments, the interaction between NcSnRK1 and NcRbohC1 was confirmed. The above results indicate that NcSnRK1 may phosphorylate certain N-terminal sites of NcRbohC1 after pollination in waterlily, thereby activating ROS burst in the stigmatic papilla cells, disrupting ROS homeostasis on stigma of waterlily, and consequently inhibiting pollen germination on the stigma. The research results can provide theoretical basis for taking effective measures to overcome pre-fertilization barrier and germplasm innovation in waterlily.
Authors: Jing Cao, Xin-Rui He, Fan-Rui Min, Chun-Qing Sun
Institution: Nanjing Agricultural University
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7225206213