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Protocol for generating nociceptor-enriched peripheral sensory neurons from human induced pluripotent stem cells

Year: 2026

Type: article

Abstract: Human induced pluripotent stem cells (hiPSCs) are a valuable system for studying human development, modeling disease-associated cellular phenotypes, and generating difficult-to-access cell types, such as sensory neurons (SNs). Here, we present a stepwise differentiation protocol for generating peripheral SNs from hiPSCs under defined culture conditions. We describe steps for expansion of hiPSCs, induction of neural crest cells (NCCs), dissociation and replating of NCCs, and maturation into SNs. This protocol yields nociceptor-enriched SN cultures.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104944

Authors: Arslan Ghazanfar, James P. Koch, Thomas Wartmann, Ahmed Y. Sanin, Sebastian Samer, Muhammad Irfan Siddique, Ulrike Steinmann, Christoph Hoeschen, Roland S. Croner, Ulf D. Kahlert

Institutions: Otto-von-Guericke-Universität Magdeburg, Leibniz Institute for Neurobiology, Northern Border University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

OpenAlex ID: W7225267813

FWCI:

Cites: 5

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