Work

Collection and cryopreservation of iliac crest bone marrow aspirate and bone cores v1

Year: 2026

Type: preprint

Abstract: This protocol describes collection of iliac crest bone marrow aspirate and solid bone cores from consenting participants undergoing routine, non-cancerous intra-abdominal surgery for the SenNet project under IRB protocol 202108156. Specimens are transported fresh to the laboratory. Bone cores are flash-frozen in liquid nitrogen and stored at −80 °C; aspirate-derived mononuclear cells are prepared for cryopreservation at 10 × 10^6 cells/mL and stored at −80 °C.

DOI: 10.17504/protocols.io.p2l64kzwkvqe/v1

Authors: Xiang Li, Daniel Rapp

Institution: Washington University in St. Louis

Language: English

Topic: Hematopoietic Stem Cell Transplantation

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7225326455

Cites:

Cited by:

Related to: 10