Work

Short-read whole-genome sequencing identifies the Tg(Tal1-cre/ERT)42-056Jrg transgene insertion site on mouse chromosome 4

Year: 2026

Type: preprint

Abstract: Tg(Tal1-cre/ERT)42-056Jrg, also known as HSC-Scl-CreERT, is a well-used transgenic allele that enables genetic manipulation in mouse hematopoietic cells. Using short-read whole-genome sequencing, we identified the transgene insertion site on chromosome 4 approximately 6 kb upstream of Echdc2.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.11.04.764251

Authors: Xiaodi Wu, Maya C. Bagish, Pablo Sánchez Vela, Alexander G. Goglia, Ross L. Levine

Institution: Memorial Sloan Kettering Cancer Center

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Memorial Sloan-Kettering Cancer Center, Cycle for Survival, Marie-Josée and Henry R. Kravis Center for Molecular Oncology, National Cancer Institute

Awards: G2051006376, G289723095, G5109122429, G7136384868