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Extraction Procedures Reshape the Detectable Fumonisin Chemical Space of Fusarium verticillioides across Culture Media

Year: 2026

Type: preprint

Abstract: Fusarium verticillioides produces fumonisins and numerous secondary metabolites, yet extraction procedures may strongly shape their analytical detection. We combined targeted and untargeted LC–HRMS approaches to compare ethyl acetate, acidified ACN/H₂O, and sequential ACN/H₂O extraction across four culture media. Extraction-dependent metabolite profiles varied markedly with both culture medium and metabolite structure. Acidified ACN/H₂O procedures generated the most consistent fumonisin B1 (FB1) signals, whereas ethyl acetate yielded weak FB1 responses but revealed complementary subsets of metabolites, including fusarin A, fusaric acid, bikaverin-related compounds, and several fumonisin-related features. Structurally related fumonisins displayed heterogeneous solvent-dependent responses, and sequential extraction provided no systematic advantage over single ACN/H₂O extraction. These findings demonstrate that extraction procedures reshape the detectable chemical space of F. verticillioides and support objective-driven solvent selection for fungal metabolomics and mycotoxin profiling.

Source: Research Square

DOI: 10.21203/rs.3.rs-11426397/v1

Authors: Emie Groppi, Térance Mobarak, Alice Gadea, Marieke Vansteelandt, Valérie Cristofoli, Mohamed Haddad

Institutions: Université de Toulouse, Institut de Recherche pour le Développement

Language: English

Topic: Mycotoxins in Agriculture and Food

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funder: Agence Nationale de la Recherche

Award: Tracing the events that turn an endophytic plant-associated fungi into a pathogen