Year: 2026
Type: preprint
Abstract: This protocol describes the installation and use of CryoNet.Refine for density-guided refinement of atomic models against cryo-electron microscopy maps. It covers software setup, preparation of an aligned starting model and density map, execution of refinement, and inspection and validation of the resulting model. A human CNT3 example demonstrates the workflow and produces a refined mmCIF model for comparison with the starting structure. Users can then apply the same procedure to their own aligned model–map pairs.
Author: Fuyao Huang
Institutions: State Key Laboratory of Membrane Biology, Tsinghua University
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7225412953