Work

High-Throughput Screening and Fragment-Based Approach for Flavivirus Antiviral Discovery

Year: 2026

Type: article

Abstract: Members of the genus Orthoflavivirus pose severe global health threats with substantial morbidity and mortality. No specific antiviral therapies are currently approved, leaving clinical management limited to supportive care. The development of broad-spectrum pan-flavivirus antivirals requires targeting highly conserved structural and non-structural proteins essential for viral replication. High-throughput screening (HTS) and fragment-based drug discovery (FBDD) represent the primary complementary methodologies for initial hit identification. This review critically compares HTS and FBDD workflows, hit rates, target strategies, and overall efficacy in pan-flavivirus drug discovery.

Source: Microorganisms

DOI: 10.3390/microorganisms14112556

Authors: Bruna Campestrini Casarin, Bernhard Ellinger, Alessandro Silva Nascimento, Carsten Wrenger

Institutions: Universidade de São Paulo, Fraunhofer Institute for Translational Medicine and Pharmacology, Instituto de Física de São Carlos, Institut Pasteur de São Paulo

Language: English

Topic: Mosquito-borne diseases and control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,258