Diagnostic Efficacy of White Side Test and Endometrial Cytology for Detection of Endometritis in Repeat Breeding Mehsana Buffaloes of Banaskantha District, Gujarat
Year: 2026
Type: article
Abstract: This study evaluated the diagnostic efficacy of the Whiteside test and endometrial cytology for detecting endometritis in repeat-breeding Mehsana buffaloes. A total of 112 repeat-breeding Mehsana buffaloes from Banaskantha District, Gujarat, were screened for endometritis during estrus. Cervico-vaginal mucus samples were tested using the Whiteside test, and endometrial cytological samples were collected with a cytobrush and stained with Field's stain. Buffaloes with ≥8% polymorphonuclear neutrophils (PMNs) in endometrial cytology were considered positive for endometritis. The Whiteside test detected endometritis in 26 of 112 buffaloes (23.21%), whereas endometrial cytology identified 35 positive cases (31.25%). The relative diagnostic efficacy of the Whiteside test was 74.28% compared with endometrial cytology. Among WST-positive buffaloes, 42.31% exhibited mild, 34.61% moderate, and 23.08% severe endometritis. The mean PMN percentage was significantly higher (p<0.05) in buffaloes with endometritis (11.04±2.13%) than in healthy buffaloes (2.50±1.65%). Endometrial cytology detected more subclinical endometritis cases than the Whiteside test. Endometrial cytology technique proved to be a more sensitive and reliable method for detecting subclinical endometritis in repeat breeding Mehsana buffaloes, whereas the White side test served as a rapid, economical, and practical field screening tool. The combined application of both techniques can facilitate early diagnosis and improve reproductive management under field conditions.
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7225459351