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High molecular weight genomic DNA extraction from horned melon (Cucumis metuliferus) leaves for long-read sequencing. v1

Year: 2026

Type: preprint

Abstract: Horned melon (Cucumis metuliferus) has relatively few genomic resources available in the public domain. Extraction of high-molecular-weight (HMW) genomic DNA (gDNA) is essential for capturing complex, repeat-rich genomic regions to improve contiguity and enable structural variation detection through long-read sequencing. Commonly used DNA extraction methods, such as CTAB extraction, fail to produce high-quality horned melon DNA suitable for long-read sequencing. Here, we present a horned melon-specific HMW gDNA extraction protocol that may also prove useful for other recalcitrant cucurbits.

DOI: 10.17504/protocols.io.1wgb1do41vpk/v1

Authors: Edward Kiegle, Monica Demar, Catarina Lino, Patience Chatukuta

Language: English

Topic: Advances in Cucurbitaceae Research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7225782441

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