Integrating biomass production platform and identification approach for stereochemical assignment of super-carbon-chain compounds
Year: 2026
Type: article
Abstract: Super-carbon-chain compounds (SCCCs), marine long-carbon-chain polyketides featuring numerous carbon stereocenters, fall into untapped chemical space in between drug-like small molecules and biopolymers. Due to mass-material unavailability and flexible-structure complexity, the reliable stereochemical assignment of SCCCs has long remained stagnant. In this work, a modern microalgal biomass production-platform is created for pilot-scale cultivation of marine dinoflagellates to produce over 100 milligrams of minor SCCCs. The modular fragment-based identification (MFBI) approach is successfully employed to establish the relative and absolute configurations of SCCCs. As an example, 157.7 mg of a minor SCCC, named amphidiketol A, featuring a highly-flexible C 76 -linear carbon backbone and thirty-three carbon stereocenters, is obtained from Amphidinium sp. in our platform. Its hybrid architecture contains eleven distinct modular fragments. Its relative and absolute configurations are successfully established by the MFBI approach. Collectively, an undescribed paradigm for stereochemical assignment of SCCCs is presented. Nine senescence-associated protein targets of amphidiketol A in lung cancer A549 cells are identified.
Source: Science Advances
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,500
OpenAlex ID: W7225825333