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Comparative Proteomic Analysis of Streptococcus agalactiae Isolates with Distinct Virulence Phenotypes

Year: 2026
Type: article
Abstract: Streptococcus agalactiae causes important disease losses in aquaculture, yet isolates of this species can differ markedly in pathogenicity. The molecular basis of these isolate-level differences remains incompletely understood. Here, we compared two fish-derived S. agalactiae isolates, MML SA-006 and MML SA-010, by assessing their effects in giant hybrid grouper and performing a comparative proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). Under the challenge conditions used, MML SA-006 produced a more severe disease outcome than MML SA-010. Analysis of the selected gel spots identified 24 spots representing 19 distinct proteins. These proteins were classified into four broad functional groups: protein translation, folding, and stress response; RNA modification, transcriptional regulation, and nucleic acid metabolism; carbohydrate and energy metabolism; and amino acid, nucleotide, and redox metabolism. The findings describe differences in protein abundance between the two isolates and provide a basis for further investigation of their potential biological significance. Further functional studies are needed to determine whether these proteins are involved in the observed differences in disease outcomes.
Source: Fishes
DOI: 10.3390/fishes11110660
Authors: Ti-Chun Chang, Yi-Chen Lin, Shu-Chun Chuang, Shang-Pin Liu, Shao-Wen Hung, Chung-Hwan Chen, Chung-Da Yang
Institutions: National Pingtung University of Science and Technology, Kaohsiung Medical University, University of Virginia, Agricultural Technology Research Institute, National Sun Yat-sen University, Kaohsiung Medical University Chung-Ho Memorial Hospital
Language: English
Subfield: Immunology
Open Access status: gold
Estimated APC paid: $3,137
OpenAlex ID: W7225833032
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