Work

Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells

Year: 2010

Type: book-chapter

Abstract: Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61737-960-4_15

Authors: Bradley T. Estes, Farshid Guilak

Institutions: Duke University, Duke Medical Center

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute on Aging, National Institute of Arthritis and Musculoskeletal and Skin Diseases