gPRG: Toward Consensus on Glycan Analysis: Reliable Methods and Reproducibility
Year: 2011
Type: article
Abstract: The field has undertaken over the past few years a series of studies to evaluate methods for glycan analysis. There are several methods that are widely used at this time for characterization of glycoprotein glycans that appear to be generally reliable. These include (1) reductive amination followed by reversed phase chromatography, (2) reductive amination with matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS), (3) reductive amination with electrospray liquid chromatography-MS, and (4) permethylation with tandem MS. The ABRF glycoprotein research group (gPRG) is planning a study in which participant laboratories carry out glycan analysis according to protocols representing best practice in the field. The results will be presented at the 2012 ABRF meeting. The results will be useful for demonstrating the reproducibility of glycan analysis using comparable protocols. They will also highlight the extent to which different methods bias the glycan analysis results obtained. This workshop will summarize the history and use of the glycan analysis methods proposed for the 2012 study. There will be opportunity for discussion and comment on these methods.
Source: Europe PMC (PubMed Central)
Authors: Daniel Kolarich, Ron Orlando, Joseph Zaia
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W758726246