Work

Simple Devices to Facilitate the Analysis of Collagen Contraction by Cells

Year: 2000

Type: article

Abstract: BAC preparation before preparing a library.It is performed in a 96-well format, takes 2-3 h and costs $2-$3/sample, far less than the cost of preparing a library, picking and purifying template, running sequencing reactions and sequencing a test plate for a given BAC.Highly contaminated preparations are readily identified and may be removed from production.This method may therefore be a useful procedure to incorporate into the "front end" of sequencing projects that require BAC purification.

Source: BioTechniques

DOI: 10.2144/00293bm02

Authors: Arno W. Tilles, Jeanne M. Classen, Mehmet Toner, Livingston Van De Water

Institutions: Harvard University, Massachusetts General Hospital, Shriners Hospitals for Children - Boston

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: Shriners Hospitals for Children, National Institutes of Health