Computer Aided Analysis of Restriction Landmark Genomic Scanning Images from Tumor and Cell Line Models
Year: 2007
Type: article
Abstract: Two-dimensional gel electrophoresis (2-DGE) was used to comprehensively scan the whole genome of normal rat mammary tissues and methylnitrosourea (MNU)-induced rat mammary tumors with and without 4HPR administration for various genetic alterations, such as DNA amplification, homozygous deletion, loss of heterozygosity (LOH), chromosome translocation and altered DNA methylation.Computer-assisted systems were applied for spot detection, quantitation, and comparison of 2-DGE DNA patterns.By comparing normal and tumor 2-DGE profiles, 12 novel spots were detected only in mammary tumors, 10 spots were absent from tumor gel profile, 8 spots in tumor lost about half of their intensity suggesting genomic deletions, and 5 spots in tumor were found amplified.Totally, 35 reproducible altered spots on rat mammary tumors 2-DGE profiles were detected.Among them, 8 spots had significantly suppressed alterations, 12 spots were lowered by 30-40% of their incidence because of 4HPR administration.These genetic alterations may represent novel changes in cancer-related genes that are central to mammary tumorigenesis.Characterizations of the 4HPR responsive alterations are not only significant to the understanding of 4HPR chemopreventive mechanisms but also to
Author: James L. Patrick
Institution: University of Toledo
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Funder: National Cancer Institute
OpenAlex ID: W769148734