Work

Limited GADD45α expression and function in IL-1β toxicity towards insulin-producing cells.

Year: 1970

Type: article

Abstract: Growth arrest and DNA damage-inducible (GADD) 45 proteins are regulators of cell death and survival. The proinflammatory cytokine IL-1β strongly increases the level of the transcript encoding GADD45α in rat insulin-producing INS-1E cells. The activation of Gadd45α gene is clearly dependent on JNK and NF-κB activation and the synthesis of the secondary mediator nitric oxide (NO). Interestingly, the observed twelve-fold increase in the GADD45α-coding transcript level is not followed by increased expression of GADD45α at the protein level. An analysis of IL-1β toxicity in INS-1E cells overexpressing GADD45α revealed no correlation between the GADD45α protein level and the sensitivity to IL-1β toxicity. These findings suggest that the potential engagement of GADD45α in IL-1β toxicity towards beta cells is limited to the effects induced by the basal expression level of this protein.

Source: Acta Biochimica Polonica

DOI: 10.18388/abp.2013_2026

Authors: Lukasz Skalniak, Ewa Gurgul-Convey, Katarzyna Okreglicka, Anna Skalniak, Jolanta Jura

Institutions: Jagiellonian University, Medizinische Hochschule Hannover

Language: English

Topic: Pancreatic function and diabetes

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,750