Year: 2013
Type: conference-paper
Abstract: NIR polymethine cyanine dyes exhibit long wavelengths that cannot be detected by the naked eye, whereas normal biological tissues fluoresce in the visible region of the electromagnetic spectrum. Using FLARE (Fluorescence-Assisted Resection and Exploration) imaging technology, both visible and NIR light can now be superimposed and visualized simultaneously. This technology has increased research in the field of biological contrast imaging agents. Polymethine cyanine dyes have shown specificity for certain cell types, while leaving the cells of neighboring tissues unaffected. The NIR properties of these dyes allow surgeons to differentiate cell types, thus the surgeon can avoid or target such tissues. The number of carbons and functional groups on the polymethine cyanine dye can alter its λMAX and distribution coefficient, manipulating the fluorescence of the dye and where it will bind in the body. In this experiment four near-infrared (NIR) heptamethine cyanine dyes were synthesized using a multi-step synthetic approach. In vivo testing at Harvard Medical College revealed these four dyes exhibited interesting binding in the vasculature of the brain, with dye JM-1 displaying the most intensity. Synthesis, purification, characterization, and in vivo test results of the four dyes will be discussed.
Source: Digital Archive @ GSU
Author: Jordan L Malray
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W785945292