Work

Review: R28 retinal precursor cells: the first 20 years.

Year: 2014

Type: article

Abstract: The R28 retinal precursor cell line was established 20 years ago, originating from a postnatal day 6 rat retinal culture immortalized with the 12S E1A (NP-040507) gene of the adenovirus in a replication-incompetent viral vector. Since that time, R28 cells have been characterized and used for a variety of in vitro and in vivo studies of retinal cell behavior, including differentiation, neuroprotection, cytotoxicity, and light stimulation, as well as retinal gene expression and neuronal function. While no cell culture is equivalent to the intact eye, R28 cells continue to provide an important experimental system for the study of many retinal processes.

Source: Europe PMC (PubMed Central)

Author: Gail M Seigel

Institution: University at Buffalo, State University of New York

Language: English

Topic: Retinal Development and Disorders

Subfield: Ophthalmology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Eye Institute, National Cancer Institute

Awards: Center on the Microenvironment and Metastasis, Tumor initiating cells in retinoblastoma, RETINAL CELL DIFFERENTIATION--EFFECT OF IMMORTALIZATION