Year: 2005
Type: dissertation
Abstract: CHAPTER 1 presents comprehensive background information regarding all aspects addressed in this thesis.Special attention was given to literature on paternal influences on embryonic development, the role of sperm RNA, sperm chromatin and sperm functional aspects i.e. morphology and acrosomal status and size.The experimental design and all relevant methods used during the study as well as the material that were used are presented in CHAPTER 2. The results of the different techniques and evaluations are provided in CHAPTER 3. It was found that 70% of the embryos that showed no developmental potential were Y-chromosome bearing embryos.The sperm selection process for ICSI based on the approach of choosing the "best looking" spermatozoon in the ejaculate seem to provide cells that can be classified as normal based on the length width ratio set by the WHO for normal cells.The chromatin packaging quality of the sperm correlated significantly and negatively with the percentage normal cells in the ejaculates.CHAPTER 4 comprises of a general discussion of the results and short summary of the major findings during the project.The discussion section focused on the paternal influence on the embryonic development and provided a suggestion for future research that can possibly lead to the use of X-chromosome bearing sperm in case of severe male factor cases.CHAPTER 5 contains the bibliographical information of the study.OPSOMMING HOOFSTUK 1 bied 'n volledige literatuu oorsig van alle aspekte wat moontlik 'n rol spell tydens embrio ontwikkeling.Daar word ook spediale aandag geskenk aan literatuur wat handel oor die vaderlike invloed, sperm RNS, kromatienpakking en funksionele aspekte soos spermsel morfologie, akrosoom status en grootte.HOOFSTUK 2 verskaf die eksperimentele studie ontwerp en all metodes asook materiaal wat tydens die projek gebruik is.Die resultate word in HOOFSTUK 3 aangebied.Daar is gevind dat 70% van die embrios wat nie verder ontwikkel het nie,kromosomm draende embrios was.Dit blyk ook dat die sperm seleksie metode wat tydens die ICSI prosedure gebruik is, naamlik die "mees geskikte" sperm, voldoende was.Die ICSI geselekteerde sperms het voldoen aan die Wêreldgesondheids Organisasie se lengte:breedte riglyne vir normale spermatozoa.Kromatienpakkings kwaliteir het betekenisvol en negatief gekorreleer met die persentasie normal spermatozoa.In HOOFSTUK 4 word die werk en resultate bespreek.Verder is daar ook 'n kort opsomming van die belangrikstre afleidings en toekoms ontwikkelings.'n Voledige bibliografiese lys word in HOOFSTUK 5 aangebied.v DEDICATION I proudly dedicate this thesis to my loving family.
Author: Kimenthra Raja
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W803463844