The Human Papillomavirus Type 16 E5 Protein: Stress Signaling and Expression in Cervical Cancer Cell Lines
Year: 2012
Type: dissertation
Abstract: Human papillomavirus (HPV) is the causative agent of 99% of cervical cancers, approximately 60% of which are caused by the high-risk type 16.While two of the three HPV-16 oncoproteins, E6 and E7, have been well characterized, the biological functions of the E5 protein remain elusive.HPV-16 E5 protein (16E5) is thought to be disrupted during viral integration, a key event in cellular transformation, suggesting that 16E5 is not essential for carcinogenesis.Conversely, its presence has been shown to enhance E6 and E7-induced transformation, and several studies, including those by Sherman et. al. (1992) and Bauer-Hofmann et. al. (1996), have shown the presence of potentially E5-coding transcripts even in cervical cancer cell lines with integrated viral genomes.However, direct confirmation of 16E5 expression in cancer cells has not been possible, mostly attributable to the lack of specific antibodies.In this thesis, we use mass spectrometry to identify 16E5 in two HPV-16 positive cervical cancer cell lines, SiHa and CaSki.This is the first time that 16E5 has been identified in cervical I. INTRODUCTION
Author: Sawali R. Sudarshan
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W827388229