Flexibility-based predictions of folding cores:characterization of the cyclophilin A - cyclosporin A complex
Year: 2015
Type: article
Abstract: Determining the folding core of a protein yields information about its folding process and dynamics. The experimental procedures for identifying the amino acids that make up the folding core include hydrogen-deuterium exchange and F-value analysis and can be expensive and time consuming. Because of this, there is a desire to improve upon existing methods for determining protein folding cores theoretically. We have obtained HDX data for the complex of cyclophilin A with the immunosuppressant cyclosporin A. We compare these data, as well as literature values for uncomplexed cyclophilin A, to theoretical predictions using a combination of rigidity analysis and coarse-grained simulations of protein motion. We find that in this case, the most specific prediction of folding cores comes from a combined approach that models the rigidity of the protein using the First software suite and the dynamics of the protein using the FRODA tool.
Authors: Heal, Jack W., Wells, Stephen A., Blindauer, Claudia A., Freedman, Robert B., Roemer, Rudolf A.
Institution: University of Warwick
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W901472995