Production of alkaline protease by free and immobilised cells of Vibrio sp under different fermentation systems and its application on deprotenisation of Prawn shell waste for Chitin recovery
Year: 2005
Type: dissertation
Abstract: This thesis presents a detailed account of a cost - effective approach\ntowards enhanced production of alkaline protease at profitable levels using\ndifferent fermentation designs employing cheap agro-industrial residues. It\ninvolves the optimisation of process parameters for the production of a\nthermostable alkaline protease by Vibrio sp. V26 under solid state,\nsubmerged and biphasic fermentations, production of the enzyme using cell\nimmobilisation technology and the application of the crude enzyme on the\ndeproteinisation of crustacean waste.The present investigation suggests an economic move towards\nImproved production of alkaline protease at gainful altitudes employing\ndifferent fermentation designs utilising inexpensive agro-industrial residues.\nMoreover, the use of agro-industrial and other solid waste substrates for\nfermentation helps to provide a substitute in conserving the already dwindling\nglobal energy resources. Another alternative for accomplishing economically\nfeasible production is by the use of immobilisation technique. This method\navoids the wasteful expense of continually growing microorganisms. The\nhigh protease producing potential of the organism under study ascertains\ntheir exploitation in the utilisation and management of wastes. However,\nstrain improvement studies for the production of high yielding variants using\nmutagens or by gene transfer are required before recommending them to\nIndustries.Industries, all over the world, have made several attempts to exploit\nthe microbial diversity of this planet. For sustainable development, it is\nessential to discover, develop and defend this natural prosperity. The\nIndustrial development of any country is critically dependent on the intellectual\nand financial investment in this area. The need of the hour is to harness the\nbeneficial uses of microbes for maximum utilisation of natural resources and\ntechnological yields. Owing to the multitude of applications in a variety of\nindustrial sectors, there has always been an increasing demand for novel\nproducers and resources of alkaline proteases as well as for innovative\nmethods of production at a commercial altitude. This investigation forms a\nhumble endeavour towards this perspective and bequeaths hope and\ninspiration for inventions to follow.
Author: Maya Paul T
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W934346210