Work

Identification of Limulus polyphemus haemocyanin messenger RNA

Year: 1981

Type: article

Abstract: Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

Source: Biochemical Journal

DOI: 10.1042/bj1960653

Authors: E J Wood, J Bonaventura

Institutions: University of Leeds, Duke University

Language: English

Topic: Hemoglobin structure and function

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, American Heart Association, Royal Society, National Institute of Environmental Health Sciences

Award: Acquisition of a Cesium Irradiator