Work

GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila

Year: 2000

Type: article

Abstract: the efficiency of transfection.In light of the fact that ECs are difficult to transfect, the success of this method is warranted for a wider range of cells with different origins.In fact, we have obtained similar results in temporally transfecting NIH 3T3 cells using this method (data not shown).In addition, this method of temporal transfection might have utility in reversing cellular abnormalities through genetic intervention of the temporally introduced gene.

Source: BioTechniques

DOI: 10.2144/00294bm10

Authors: Scott Barolo, Lucy A. Carver, James W. Posakony

Institution: University of California San Diego

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415