Characteristics of the released single cells from colonial oil-producing green alga Boryococcus braunii and expression cassettes for transformation
Year: 2014
Type: dissertation
Abstract: useful material for molecular engineering, such as, cell fusion or genomic transformation.I made several nuclear transformation constructs that are composed of a domestic promoter, marker gene ORF and a terminator.As a marker gene, I used bacterial phlenomycin (ble), fused gene of ble and codon optimized GFP or GUS reporter gene.In addition to above, two constructs was made for UTEX572 plastid transformation.In this study, I also showed that a mesoporous metal oxide (TiO2) with a density of approximately only one-tenth that of gold or tungsten have potency to be effective as a DNA carrier in biolistic bombardment of a rigid cell wall-containing alga.This new particle might be a good material with wide application prospects.Two peaks of gas pressures in the transformation ratio were detected irrespective of whether the particles were made of gold, tungsten, or TiO2.
Author: HOU, Liyuan
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W950127792