Year: 2006
Type: article
Abstract: Prostate cancer is the most commonly diagnosed cancer except skin cancer in the United Stares.Each year, 70,000 men require additional treatment due to a recurrence of prostate cancer and the cause of prostate cancer death is by cancer metastasis.Gossypol, a natural polyphenolic compound present in cottonseeds, possesses antiproliferative and pro-apoptotic effects in various cancer cells.The (-)-gossypol enantiomer is a more potent inhibitor of cancer cell growth than (+)-or (±)-gossypol.In this dissertation, we hypothesize that (-)-gossypol serves as a chemopreventive agent through suppressing cell growth, inducing apoptosis and reducing metastasis in prostate cancer cells.First, we demonstrated that (-)-gossypol reduced cell viability in human primary cultured prostate cancer epithelial cells, and human prostate cancer cell lines as well as rat prostate cancer cells.The growth inhibition caused by (-)-gossypol treatment was associate with down-regulation of cyclin-D1 and Rb, but with up-regulation of p21 and TGF-β1 expression at mRNA and/or protein levels.Second, cancer cells treated with (-)-gossypol induced programmed cell death, apoptosis.This induction of cell death was measured by DNA fragmentation, and was related to the Bcl-2 family of apoptosis, mainly regulated mitochondrial cell death pathway.(-)-Gossypol treatment inhibited the anti-apoptotic Bcl-2 and Bcl-xL protein expression, but increased the pro-apoptotic vii
Author: Yi‐Wen Huang
Institution: The Ohio State University
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W962323442