The 1961 Middletown Oral Poliovirus Vaccine Program. IX. Isolation of enteroviruses from sewage before and after vaccine administration.
Year: 1962
Type: article
Abstract: In conjunction with the city-wide program of oral vaccination conducted during the early months of 1961 in Middletown, Connecticut, tests were carried out on serial samples of sewage from various strategic points in the city as a means of virological surveillance. Objectives were: a) to determine the relative prevalence of polioviruses and other enteroviruses in the city sewage prior to, during, and subsequent to the vaccination campaign; b) to continue attempts to isolate enteroviruses from sewage for at least one year in an effort to determine how long the attenuated poliovirus vaccine strains might persist in sewers servicing communities of different sizes. METHODS Collection of sewage. Six sewage sampling points were selected, five throughout the city, and one in the adjacent town of Portland which served somewhat as a control. The areas from which the sewage came are illustrated in the map in Figure 1. The sampling points which were either in branch sewers or at the influent of sewage treatment plants, were designated as follows: area no. 1 (Green Street pumping station) which carried sewage from northern and northwestern parts of the city, servicing approximately 8,000 people; area no. 2 (Middletown sewage treatment plant) represented sewage not only from the center of the city but, with one exception, from all of the other sampling points of the city, servicing approximately 20,000 people; area no. 3 (the south interceptor line) carried sewage from the southern part of the city, servicing approximately 7,000 people; area no. 4 sewage came from a small group of houses representing approximately 300 people (the effluent of this line was independent of the city sewage-treatment plant); area no. 5 carried the sewage from a correctional institution with a population of 160 teenagers; and area no. 6
Source: PubMed Central
Author: J T RIORDAN
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W974891442