Year: 2006
Type: article
Abstract: CHROMATIN IMMUNOPRECIPITATION (CHIP) 2.1.ABSTRACT……………………………………………………………………..42 2.2.INTRODUCTION………………………………………………………………43 2.3.MATERIALS AND METHODS 2.3.1.Cell culture and treatment…………………………………………………45 2.3.2.MOLT-4 expression profiling with cDNA arrays………………………...46 2.3.3.Chromatin Immunoprecipitation Assay (ChIP) …………………………..48 2.4.RESULTS 2.4.1.Expression profiling identifies radiation responsive genes………………..52 2.4.2.Identification of p53 binding sites in radiation-responsive genes implicated in apoptosis and cell cycle control………………………55 2.4.3.Chromatin immunoprecipitation (ChIP) of p53 with regulatory regions of radiation-responsive genes implicated in apoptosis and cell cycle control………………………………………………………57 2.5.DISCUSSION…………………………………………………………………...64
Author: Meredith E. Crosby
Institution: Case Western Reserve University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W98571078