Author

Thomas P. Graycar

Observed names: T Graycar, T P Graycar, T. Graycar, T. P. Graycar, T.P. Graycar, THOMAS GRAYCAR, THOMAS P. GRAYCAR, Thomas Graycar

Observed institutions: Genentech, University of Toronto

OpenAlex ID: A5067812218

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

1985 · D A Estell, T P Graycar, et al. · Journal of Biological Chemistry · 337

Probing Steric and Hydrophobic Effects on Enzyme-Substrate Interactions by Protein Engineering

1986 · D. A. Estell, T. P. Graycar, et al. · Science · 247

Designing substrate specificity by protein engineering of electrostatic interactions.

1987 · J A Wells, D B Powers, et al. · Proceedings of the National Academy of Sciences · 199

The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.

1988 · R Bott, M Ultsch, et al. · Journal of Biological Chemistry · 177

Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering.

1987 · J A Wells, B C Cunningham, et al. · Proceedings of the National Academy of Sciences · 134

Importance of hydrogen-bond formation in stabilizing the transition state of subtilisin

1986 · J. A. Wells, B. C. Cunningham, et al. · Philosophical Transactions of the Royal Society of London Series A Mathematical and Physical Sciences · 133

Alteration of the specificity of subtilisin BPN' by site-directed mutagenesis in its S1 and S1' binding sites

1991 · Pierre R. Bonneau, Thomas P. Graycar, et al. · Journal of the American Chemical Society · 59

Protein and solvent engineering of subtilisin BPN' in nearly anhydrous organic media

1993 · Pramod P. Wangikar, Thomas P. Graycar, et al. · Journal of the American Chemical Society · 58

Chemical Modification of Cysteine Mutants of Subtilisin Bacillus lentus Can Create Better Catalysts Than the Wild-Type Enzyme

1997 · Per Berglund, Grace DeSantis, et al. · Journal of the American Chemical Society · 41

Probing the specificity of the S1 binding site of M222 mutants of subtilisin B. lentus with boronic acid inhibitors

1996 · Michele R. Stabile, W. George Lai, et al. · Bioorganic & Medicinal Chemistry Letters · 35

Engineered Bacillus lentus subtilisins having altered flexibility 1 1Edited by P. E. Wright

1999 · Thomas Graycar, Mark Knapp, et al. · Journal of Molecular Biology · 29

Subtilisins

2012 · Thomas P. Graycar, Richard R. Bott, et al. · Handbook of Proteolytic Enzymes · 21

Altering the specificity of subtilisin B. Lentus by combining site-directed mutagenesis and chemical modification

1996 · Per Berglund, Michele R. Stabile, et al. · Bioorganic & Medicinal Chemistry Letters · 18

Altering the Proteolytic Activity of Subtilisin through Protein Engineering

1992 · THOMAS P. GRAYCAR, RICHARD R. BOTT, et al. · Annals of the New York Academy of Sciences · 14

The Effects of Organic Solvents on Wild-Type and Mutant Subtilisin-Catalyzed Hydrolyses

1993 · P.R. Bonneau, M. Eyer, et al. · Bioorganic Chemistry · 9

Protein Engineering of Subtilisin

1991 · Thomas P. Graycar · Springer eBooks · 9

Importance of Conformational Variability in Protein Engineering of Subtilisin

1987 · Richard Bott, Mark Ultsch, et al. · ACS symposium series · 8

Using Structural Comparison as a Guide in Protein Engineering

1992 · RICHARD BOTT, JUDY DAUBERMAN, et al. · Annals of the New York Academy of Sciences · 7

On the Evolution of Specificity and Catalysis in Subtilisin

1987 · J.A. Wells, B.C. Cunningham, et al. · Cold Spring Harbor Symposia on Quantitative Biology · 7

Proteolytic Cleavage, Reaction Mechanisms

2009 · Wolfgang Aehle, Richard Bott, et al. · Encyclopedia of Industrial Biotechnology · 6

Packing selection of Bacillus lentus subtilisin and a site-specific variant

1994 · J. L. Dauberman, G. Ganshaw, et al. · Acta Crystallographica Section D Biological Crystallography · 6

Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus Lentus Subtilisin

1996 · Richard Bott, Judy Dauberman, et al. · Advances in experimental medicine and biology · 2

Engineering Altered Substrate Specificity into Subtilisin BPN′

1991 · Richard Bott, Thomas Graycar, et al. · Springer eBooks · 2

Contributors

2012 · Catherine Anne Abbott, Carmela R. Abraham, et al. · Handbook of Proteolytic Enzymes · 1

Tempering with success: using structure to develop second-generation engineered subtilisins

1993 · R. Bott, J. Dauberman, et al. · Acta Crystallographica Section A Foundations of Crystallography · 1

PDF

Works count: 29

Citation count: 1,560

H-index: 14

I10-index: 14