Development of refined detection system for mature and active IL-1b family proteins
Description: Inflammation is a common risk factor for common diseases, such as cancer, and autoimmune disease. Interleukin‐1b and IL-18 are pro-inflammatory cytokines assigned to the IL-1 family because of its structural homology, receptor utilization and signal transduction pathways. Both of IL-1b and IL-18 are initially synthesized as inactive precursors and the cleavage required for processing into mature forms is mainly mediated by pro-inflammatory caspases following the activation and assembly of macromolecular complexes called inflammasomes. Detection of these neoepitopes of mature forms should provide a unique and sensitive indicator of inflammatory action.A refined detection system for mature and active IL-1b and IL-18 could be developed by utilizing our developed neoepitope mAbs in combination with epitope-different mAbs. Such a system might be useful in inflammasome biology, and for diagnostic and therapeutic strategies for inflammatory diseases.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2017-04-01
End date: 2020-03-31
Investigator: Urano Takeshi (lead)
Institution: Shimane University
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 17
Funder award ID: 17K08983
OpenAlex ID: G10080705