The preparation of gene knock out tick by TALEN using nuclease for infectious model
Description: Although tick studies were conducted by gene silencing using RNA interferance for analyzing the gene function of tick, the skills of gene knockeout and gene transgenic for ticks have not been developing at the morment.Therefore, we tried to incorporate foreign gene into the tick cell line for establishing gene enginering. Although the effect of gene plasmid transfeciton into the tick cell line showed high luceferase activity using pmiGLO or pCAGGS-Luceferase,the effect of plasmid transfection into the tick cell line showed low luceferase activity using actin or ferritin pomotor.These studies indicated that the foreign gene incorporated into the tick may make a break through the praparation of gene konockout or trangenic tick.
Amount: ¥3,900,000
Funding type: research
Funder scheme: Grant-in-Aid for Challenging Exploratory Research
Start date: 2014-04-01
End date: 2016-03-31
Investigator: TETSUYA TANAKA
Institution: Kagoshima University
Funded outputs count: 14
Funder award ID: 26660229
OpenAlex ID: G1020143082