Award

High-Throughput Measurements of RNA-Mediated Regulation of Gene Expression and Oncogenesis

Description: Project Narrative RNA-binding proteins (RBPs) control most aspects of post-transcriptional gene regulation and cause several types of human cancers when mutated, which disrupts their interactions with many different RNAs. Currently, it is not well understood how RBPs are organized to regulate the expression of the RNAs they bind, and which specific domains within them have regulatory potential that is sensitive to mutation. In this work, I will develop and use a high-throughput recruitment assay to identify functional ‘effector’ domains within RBPs, link them to their molecular and cellular mechanisms of regulating RNA, and determine how mutations within them lead to cancer developmen.

Funder: National Institutes of Health

Amount: $51,240

Funding type: fellowship

Funder scheme: F30

Start date: 2024-04-01

End date: 2027-03-31

Investigator: ABBY THURM

Institution: STANFORD UNIVERSITY

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences