New high performing microscale modalities of MALDI MS/MS
Description: New developments in miniaturised MALDI MS/MS sample processing schemes both in biofluid processing as well as in high resolution tissue MALDI imaging will be performed. Based on our previous development of a MALDI target integrated solid phase extraction micro array, ISET, a new dimension will be developed enabling immuno affinity extraction of target proteins or peptides from complex biofluids directly on the ISET. We have recently developed a protocol for performing microbead based reverse phase protein capture, reduction, alkylation and purification, analyte crystallisation and MALDI MS readout all in an unbroken sequence on the same microchip. The further development of this protocol will now also include the initial immobilisation of antibodies on the solid phase as well as further assay optimisation. This development will open the route to biomarker screening in biobank repositories.. An optional route will also be developed utilising protein specific aptamers as the capture probe. Protocols for aptamer activation of solid phase microbeads or immobilisation in high surface area sol-gel matrices will be developed and utilised for the protein capture and subsequent processing prior to MALDI MS/MS analysis. We will also develop state of the art high speed MALDI matrix spotting for tissue MALDI imaging where high spatial resolution of drug compound localisation in tissue will be mapped.
Funder: Vetenskapsrådet
Amount: SEK 3,900,000
Funding type: research
Funder scheme: Project grant
Start date: 2009-01-01
End date: 2011-12-31
Investigator: Thomas Laurell (lead)
Institution: Lund University
Funded outputs count: 5
Funder award ID: 2009-05361
OpenAlex ID: G1764062096