Development of single-molecule measurement with angstrom precision
Description: We have developed an optical microscopic single-molecule method based on the scattering imaging of plasmonic metal nanoparticles, and achieved atomic-level (angstrom-level) localization precision and microsecond time resolution. In addition, the developed method has been successfully applied to linear and rotary molecular motors such as kinesin and V1-ATPase to elucidate their chemo-mechanical coupling mechanism and elementary process of motion in detail. Furthermore, we have developed a dual-color, high-speed/high-precision single-molecule measurement method using silver and gold nanoparticles as probes, and a method estimating chemical state-dependent free energy profile from the single-molecule trajectory of the movement of molecular motor.
Amount: ¥17,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (B)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Ryota IINO (lead)
Institution: Institute for Molecular Science
Funded outputs count: 6
Funder award ID: 18H02418
OpenAlex ID: G1906294249